rabbit anti endostatin  (Boster Bio)

Journal: Cellular & Molecular Biology Letters

Article Title: Limbal niche cells can reduce the angiogenic potential of cultivated oral mucosal epithelial cells

doi: 10.1186/s11658-018-0133-x

Figure Lengend Snippet: Primer sequences used for RT-qPCR

Article Snippet: Rabbit anti-endostatin antibody , BIOSS (BS-0547R) , 1:300.

Techniques: Sequencing

Journal: Cellular & Molecular Biology Letters

Article Title: Limbal niche cells can reduce the angiogenic potential of cultivated oral mucosal epithelial cells

doi: 10.1186/s11658-018-0133-x

Figure Lengend Snippet: Antibodies used in western blotting

Article Snippet: Rabbit anti-endostatin antibody , BIOSS (BS-0547R) , 1:300.

Techniques: Western Blot

Journal: Cellular & Molecular Biology Letters

Article Title: Limbal niche cells can reduce the angiogenic potential of cultivated oral mucosal epithelial cells

doi: 10.1186/s11658-018-0133-x

Figure Lengend Snippet: Expression of angiogenesis-related proteins of cultivated oral mucosal epithelial cells (COMECs) co-cultured with LNCs and 3 T3 cells. a Relative protein expression levels of bFGF, PEDF, VEGF, TSP-1, TIMP-3, and endostatin were detected by western blotting normalized with GAPDH. b Densitometric analysis of specific immunoreactive bands was performed. c The sFlt-1 concentrations in the conditioned medium were detected by enzyme-linked immunosorbent assays. The results were expressed as mean ± standard deviation. LNCs: limbal niche cells, bFGF: basic fibroblast growth factor, PEDF: pigment epithelium-derived factor, VEGF: vascular endothelial growth factor, TSP-1: thrombospondin-1, TIMP-3: tissue inhibitor of metalloproteinase-3, GAPDH: glyceraldehyde 3-phosphate dehydrogenase, sFlt-1: soluble fms-like tyrosine kinase-1, *: p < 0.05

Article Snippet: Rabbit anti-endostatin antibody , BIOSS (BS-0547R) , 1:300.

Techniques: Expressing, Cell Culture, Western Blot, Standard Deviation, Derivative Assay

Journal: Journal of Cancer

Article Title: Oridonin inhibits tumor angiogenesis and induces vessel normalization in experimental colon cancer

doi: 10.7150/jca.55929

Figure Lengend Snippet: ORI inhibited angiogenesis of colon cancer. (A) Representative images of immunohistochemical analysis of tumor microvessels (×200, scale bar =100 µm). (B) CD31 + MVD was dramatically suppressed in ORI- treated group compared to the Control group. (C) Representative Western blot expressions of VEGF, bFGF, angiostatin and endostatin. (D and E) The levels of VEGF and bFGF were dramatically down-regulated in the ORI-treated group, while (F and G) angiostatin and endostatin were increased by treatment with ORI. * p < 0.05, ** p < 0.01.

Article Snippet: The membranes were incubated with primary antibodies as follows: rabbit anti-JAK2 (1:1000, Cell Signaling Technology), or rabbit anti-p-JAK2 (1:1000, Cell Signaling Technology), or mouse anti-STAT3 (1:1000, Cell Signaling Technology), or mouse anti-p-STAT3 (1:2000, Cell Signaling Technology), or rabbit anti-β-actin (1:6000, Abcam), or rabbit anti-VEGF (1:1000, Servicebio), or rabbit anti-bFGF (1:1000, Bioworld), or rabbit anti-angiostatin (1:1000, Bioworld), or rabbit anti- endostatin (1:1000, Boster), with gentle shaking at 4 °C overnight.

Techniques: Immunohistochemical staining, Control, Western Blot

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Proteomic Screening of Anaerobically Regulated Promoters from Salmonella and Its Antitumor Applications *

doi: 10.1074/mcp.M111.009399

Figure Lengend Snippet: The adhE promoter anaerobically effectively drove the expression of GFP or the secretion of endostatin in S. typhimurium. A, Strategy used for construction of single-copy GFP fusion. The adhE-GFP fusion was integrated into the VNP chromosome by homologous recombination. B, Fluoroscopy analysis of mutVNP-GFP grown under anaerobic and aerobic conditions. Only bacteria grown under anaerobic condition showed green fluorescence. Fluorescence intensity of mutVNP-GFP was determined and the values in the bars are the median fluorescence intensities of all individual bacteria in the populations. (Mean ± S.D., n = 3, †p < 0.001). C, Schematic diagram of the endostatin expression vector. D, Western blot analysis with rhEndostatin-specific antibodies to detect rhEndostatin in extracellular and intracellular fractions of VNPpadhEEndostatin.

Article Snippet: Rabbit-anti-endostatin polyclone antibody (Santa Cruz Biotechnology, Santa Cruz, CA) was used for detecting the expression of endostatin.

Techniques: Expressing, Homologous Recombination, Fluorescence, Plasmid Preparation, Western Blot